Regulatory

Part:BBa_K117002:Design

Designed by: Nguyen Xuan Hung   Group: iGEM08_NTU-Singapore   (2008-10-07)
Revision as of 04:48, 29 October 2008 by Dariusaw (Talk | contribs) (→‎Design Notes)

LsrA promoter (indirectly activated by AI-2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

LsrA is initially commercially synthesized with RBS BBa_B0034 by ATIbiotech in a blue script cloning vector. LsrA is then extracted using PCR with oligo fragments of DNA sequence 5'-GAATTATGTATACATAATGGCA-3' & 5'-GGTAAATATCCTTTAATTAAA-3' with Biobricks prefix and suffix respectively.

References