Regulatory
Part:BBa_K123003
Designed by: Jason Gardiner Group: iGEM08_University_of_Alberta (2008-10-27)
ER
This basic part is derived from the Human Estrogen Receptor Alpha and optimized for activity in E.Coli. When estrogen or an estrogen like compound is present the receptor binds to it and forms a homodimer. This homodimer is then able to bind to an Estrogen Responsive Element (ERE BBA_K123002). In Humans this estrogen receptor (ER) is a transcription factor that inciates transcription. However because we are interested in using this protien in bacteria we could not assume the same enhancer like effects. We have designed this part to act as a repressor when bound to the ERE by physically blocking the Polymerase
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 24
Illegal NgoMIV site found at 902
Illegal AgeI site found at 870
Illegal AgeI site found at 1841 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |