Reporter
Part:BBa_K3338001:Design
Designed by: Jonas Scholz Group: iGEM20_Hannover (2020-10-22)
Human codon optimized Gaussia luciferase hGLuc
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence of the Gaussia Luciferase is codon optimized for expression in human cells.
Source
The sequence was purchased from Addgene (Plasmid #22522) (Pastrana et al. 2009).
References:
Pastrana, D. V., Tolstov, Y. L., Becker, J. C., Moore, P. S., Chang, Y., & Buck, C. B. (2009). Quantitation of human seroresponsiveness to Merkel cell polyomavirus. PLoS pathogens, 5(9), e1000578.