Composite

Part:BBa_K3328026:Design

Designed by: Qi Wu   Group: iGEM20_OUC-China   (2020-10-04)
Revision as of 16:19, 20 October 2020 by Felix Felicis (Talk | contribs) (→‎Design Notes)


triggers of NIMPLY gate (NIMPLY2)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 110
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 107
    Illegal XhoI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 110
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 110
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In the NIMPLY gate, a deactivating RNA (INPUT A) uses direct hybridization or strand displacement to abolish trigger RNA (input B) activity. So when only the correct trigger RNA is expressed, the switch can be turned on.

Source

synthesize from company


References

[1] Green, A. A., Kim, J., Ma, D., Silver, P. A., Collins, J. J., & Yin, P. (2017). Complex cellular logic computation using ribocomputing devices. Nature, 548(7665), 117–121. doi:10.1038/nature23271

[2] Green, A. A., Silver, P. A., Collins, J. J., and Yin, P. (2014) toehold switches: de-novo- designed regulators of gene expression. Cell 159, 925– 939, DOI: 10.1016/j.cell.2014.10.002