Composite

Part:BBa_K3328014:Design

Designed by: Chaoxin Chen   Group: iGEM20_OUC-China   (2020-10-04)
Revision as of 17:58, 20 October 2020 by Hester (Talk | contribs)


triggers of XOR gate (XOR1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The XOR is inspired by the NIMPLY gate, it is consisted of a toehold switch and two triggers. The trigger’s core sequence is same and at the triggers’ both ends there are the nucleotide-binding domains. When input one of these triggers, the switch can turn on. And when input these two triggers at the same time, they can pair together and form a ring in the middle, as the result, the switch will still be in OFF state.


Source

synthesize from company


References

[1] Green, A. A., Silver, P. A., Collins, J. J., and Yin, P. (2014) Toehold switches: de-novo- designed regulators of gene expression. Cell 159, 925– 939, DOI: 10.1016/j.cell.2014.10.002

[2] Green, A. A., Kim, J., Ma, D., Silver, P. A., Collins, J. J., & Yin, P. (2017). Complex cellular logic computation using ribocomputing devices. Nature, 548(7665), 117–121. doi:10.1038/nature23271