Coding

Part:BBa_K3335000

Designed by: TianYu Ma   Group: iGEM20_NJU-China   (2020-10-20)
Revision as of 05:08, 22 October 2020 by Cuber straw (Talk | contribs)


We use KIBRA to control exosome secretion

KIBRA as an adaptor-like protein that stabilizes Rab27a, which in turn controls exosome secretion. Rab27a is stabilized by interacting with KIBRA, which prevents ubiquitination and degradation via the ubiquitin-proteasome pathway. KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a[1].

In our experiment, we used KIBRA to promote cell release of exosomes containing siRNA, thus increasing the efficiency of cell production and release of exosomes and improving the killing of tumor cells.

[1]Lin Song et al,.KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a.Nature Communication,2019.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 335
    Illegal BglII site found at 2999
    Illegal BamHI site found at 246
    Illegal BamHI site found at 442
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 435
    Illegal BsaI site found at 2598
    Illegal BsaI.rc site found at 1920
    Illegal BsaI.rc site found at 2644


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Categories
Parameters
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