Composite

Part:BBa_K3578010

Designed by: Ruyi Shi   Group: iGEM20_Worldshaper-Nanjing   (2020-09-20)
Revision as of 14:51, 20 September 2020 by LucyShi 2018 (Talk | contribs)


AnGlu Starch degradation

EXP, AnGlu, XPR2t part will be digested by BsaI and then assembled as the module 1 through the T4 ligase. The module 1 and Leu selection marker will be digested by SapI and then assembled as “EXP-AnGlu-XPR2t-Leu” expression cassette through the T4 ligase. The cassette can be transformed into the Yarrowia lipolytica plotoplast and integrated into the genome for AnGlu efficient expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3683
    Illegal EcoRI site found at 3733
    Illegal EcoRI site found at 4695
    Illegal SpeI site found at 756
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3683
    Illegal EcoRI site found at 3733
    Illegal EcoRI site found at 4695
    Illegal NheI site found at 817
    Illegal SpeI site found at 756
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3683
    Illegal EcoRI site found at 3733
    Illegal EcoRI site found at 4695
    Illegal BglII site found at 2537
    Illegal BglII site found at 5964
    Illegal BamHI site found at 3157
    Illegal XhoI site found at 4678
    Illegal XhoI site found at 4707
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3683
    Illegal EcoRI site found at 3733
    Illegal EcoRI site found at 4695
    Illegal SpeI site found at 756
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3683
    Illegal EcoRI site found at 3733
    Illegal EcoRI site found at 4695
    Illegal SpeI site found at 756
    Illegal NgoMIV site found at 3821
    Illegal AgeI site found at 3364
    Illegal AgeI site found at 5292
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None