Regulatory

Part:BBa_K3408000

Designed by: Fengzhi Yuan   Group: iGEM20_NAU-CHINA   (2020-10-02)
Revision as of 04:34, 22 October 2020 by NJAU-Chappie (Talk | contribs)

promoter nar(fnr regulated)


We add the promoter “Pnar”, in response to the RES-FNR oxygen regulator system of Bacillus Subtilis, to express phy(ycD) phytase efficiently in the absence of oxygen, and meanwhile express CI repressor protein to inhibit toehold switch-Dpnc downstream.

According to the paper, they have designed three kinds of strength of the promoter Pnar and applied them in E.coli successfully. The expression level of GFP in the control of high-strength promoter was 19.7 higher than the wild-type promoter.

T--NAU-CHINA--basic-parts1.png

Fig.1. The spacer sequence of 15 bp between the - 35 and - 10 elements of the upstream region of the wild-type nar promoter was randomized(Hwang Hee Jin, et al., BIOTECHNOL BIOFUELS. 2018)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None