Part:BBa_K3398003
mamC_ZZ fusion protein
This part is encodes a fusion protein of mamC and ZZ. And for separation from other proteins at the purification step, we introduced a GST tag at the N-terminus and a thrombin site between the fusion protein and tag for cleavage after purification, which has been submitted as BBa_K3398004. To maximize the expression of a functional protein, we employed codon optimization to maximize the expression of a functional protein in Escherichia coli. Not only in E.coli, these parts can also be expressed by magnetotactic bacterium such as MSR-1 to produce magnetosomes modified with ZZ protein on the surface as a self-assembly platform for polymer varied antibodies.
Usage and Biology
MamC protein is a membrane protein which covers the most area of the magnetosome surface formed by magnetotactic bacteria MSR-1, and ZZ is the Fc-binding domain of staphylococcal protein A (SPA), an immunoglobulin-binding protein from the cell wall of Staphylococcus aureus. This fusion protein can be expressed and anchored onto the surface of magnetosomes by transferring the recombinant plasimd into MSR-1.The resulting recombinant magnetosomes will be capable of self-assembly with the Fc region of mammalian antibodies and will ve therefore useful for functionalization of magnetosomes such as being developed to a antibody vector or serving as specifically tumor-targeting contrast agents for MRI.
Characterization
Results
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 36
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