Composite

Part:BBa_K3506022

Designed by: Yiling Chen   Group: iGEM20_BNU-China   (2020-10-21)
Revision as of 15:40, 21 October 2020 by Chenyiling (Talk | contribs)


Inducible double promoter system

GAL7 promoter can be induced by galactose in Cryptococcus neoformans. It is the first inducible promoter characterized in C. neoformans. RNA polymerase III promoter of the U6 small RNA gene is a common part for eukaryotic expression systems. Pol III uniquely transcribes small non-coding RNAs, including 5S rRNA, tRNAs, and other essential RNAs such as the U6 snRNA【1】. But it is known that RNA polymerase III transcription product does not have polyA and cannot be captured by Oligo dT for information reading.We designed this composite part to read the sequence information downstream of the U6 promoter in real time and at RNA level through the drive of GAL7 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 402
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 742


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Categories
Parameters
None