Device

Part:BBa_K3402053

Designed by: Yibo Shi   Group: iGEM20_Jiangnan_China   (2020-10-21)
Revision as of 08:47, 21 October 2020 by Registry (Talk | contribs)

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Self-excising hygromycin marker cassette

This device is composed of upSBLE(BBa_K3402030), Six site(BBa_K3402032), Pgalk(BBa_K3402033), Rec(BBa_K3402034), Tgki(BBa_K3402019), hph(BBa_K3402012), Six site(BBa_K3402032), Ptef1(BBa_K3402007), Cas9(BBa_K3402023), NLS(BBa_K3402008), Tsyn7(BBa_K3402001), doSBLE(BBa_K3402031).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 858
    Illegal XbaI site found at 2596
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1380
    Illegal BamHI site found at 445
    Illegal BamHI site found at 3783
    Illegal XhoI site found at 2214
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 858
    Illegal XbaI site found at 2596
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 858
    Illegal XbaI site found at 2596
    Illegal NgoMIV site found at 523
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None