Protein_Domain
Part:BBa_K3582004:Design
Designed by: Saillesh Aravindhan C Group: iGEM20_IISER-Pune-India (2020-10-20)
The DBLb domain of PF11_0521 PfEMP1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1407
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1113
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
- 1] RFC10 criteria satisfied
- 2] 6x-His-tag gene added for purification
- 3] Codon optimized with E-coli K-12 strain
Source
Protein sequence was obtained from PDB and reverse translated to give this biobrick