Coding

Part:BBa_K2924028:Design

Designed by: Melanie Sbielut, Andreas Nakielski   Group: iGEM19_Duesseldorf   (2019-10-12)
Revision as of 15:43, 19 October 2019 by AndreasN (Talk | contribs)


β-casein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 673
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 630
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

The sequence is codon-optimized for expression in E. coli

Source

The DNA sequence was acquired by reverse-translating the protein sequence of b-casein.

References