Coding

Part:BBa_K1929300

Designed by: Maxwell Ng   Group: iGEM16_McMasterU   (2016-10-13)
Revision as of 21:09, 13 October 2016 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Interleukin-2 (IL-2) with designed RBS

Interleukin-2 (IL-2) protein coding sequence. Silent point mutation at base pair insert 272 (A>T) to make BioBrick compatible (removed an XbaI site). Designed RBS with spacer DNA added preceding the IL-2 for protein expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 630
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//collections/immune_regulation/inflammatory
Parameters
None