Device

Part:BBa_K3402052

Designed by: Yibo Shi   Group: iGEM20_Jiangnan_China   (2020-10-20)
Revision as of 12:25, 20 October 2020 by Registry (Talk | contribs)

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GFP editing expression cassette

This device is composed of promoter Ptef1(BBa_K3402007), Cas9(BBa_K3402023)，NLS(BBa_K3402008), terminator Tsyn7(BBa_K3402001), HDV(BBa_K3402028), sgGFP(BBa_K3402024), HH and RS(BBa_K3402029), Ptef1(BBa_K3402007), upGFP(BBa_K3402025), hygromycin resistance gene hph(BBa_K3402012) and doGFP(BBa_K3402026).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5609
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1736
    Illegal BglII site found at 5300
    Illegal XhoI site found at 1051
    Illegal XhoI site found at 1786
    Illegal XhoI site found at 2149
    Illegal XhoI site found at 5101
    Illegal XhoI site found at 6701
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5609
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5609
    Illegal NgoMIV site found at 2563
    Illegal NgoMIV site found at 3667
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1507
    Illegal BsaI site found at 2224
    Illegal SapI.rc site found at 7265


[edit]
Categories
Parameters
None