Composite

Part:BBa_K3329062

Designed by: Wenfei Xiao   Group: iGEM20_BNDS_China   (2020-10-20)
Revision as of 11:52, 20 October 2020 by Registry (Talk | contribs)

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SpyTag003*3-Scl2*2-sfGFP for P. Pastoris

SpyTag003 is a sub part in the Spytag-catcher003 system. It should be used with Spycatcher003. The cellulose binding module help connect cellulose and Spycatcher003. The monomeric red fluorescent protein is connected with CBM. The super-folder GFP protein is connected with scl2. The green color indicates whether the scl2 is bound to cellulose by seeing whether that filter paper turned green.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 560
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 787
    Illegal NgoMIV site found at 1116
    Illegal NgoMIV site found at 1663
    Illegal NgoMIV site found at 1695
    Illegal AgeI site found at 1492
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 862
    Illegal BsaI.rc site found at 1588


[edit]
Categories
Parameters
None