Part:BBa_K3351009

His tag.

Summary

His tags have gained great popularity over the last decade as a purification tool for recombinant proteins. Cloning vectors generally introduce six consecutive histidines and an optional protease-cleavage site or linker to the N- or C-terminus of the protein of interest. These His tags facilitate selective binding of the expressed protein to a nickel-affinity column. The tag may then optionally be removed by a protease, requiring another purification step.

Reference

[1] Smith, M. C., Furman, T. C., Ingolia, T. D. & Pidgeon, C. (1988). J. Biol. Chem. 263, 7211–7215 [2] Carson M , Johnson D H , Mcdonald H , et al. His-tag impact on structure[J]. Acta Crystallographica, 2007, 63(3):295-301. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]