Part:BBa_K3409013

Microcin PDI gene cluster

Contains all the elements necessary to constitutively express the entire Microcin PDI gene cluster to produce and secrete the antimicrobial peptide Microcin PDI: mcpM: coding for microcin PDI, mcpI: coding for the immunity protein, mcpA: coding for the activator protein, mcpD and mcpB coding for the secreting proteins. mcpM, mcpI, mcpA are under the control of the same promoter and the sequence is organized as follows: a promoter (BBa_J23111), an RBS (BBa_J61127), the CDS for McpM-His (BBa_K3409003), an RBS (BBa_J61130), the CDS for McpI (BBa_K3409004), an RBS (BBa_J61118), the CDS for McpA (BBa_K3409005) and a double forward terminator (BBa_B0015). mcpD and mcpB are under the control of another promoter and the sequence is organized as follows: a promoter (BBa_J23118), an RBS (BBa_J61118), the CDS for McpD, (BBa_K3409006), an RBS (BBa_J1109), the CDS for McpB (BBa_K3409007) and a double terminator (BBa_B0015).

Usage and Biology

Bibliography

Characterization of a novel microcin that kills enterohemorrhagic escherichia coli O157:H7 and O26. Applied and Environmental Microbiology, 78(18), 6592–6599. https://doi.org/10.1128/AEM.01067-12

Lu, S. Y., Graça, T., Avillan, J. J., Zhao, Z., & Call, D. R. (2019). Microcin PDI inhibits antibioticresistant strains of Escherichia coli and Shigella through a mechanism of membrane disruption and protection by homotrimer self-immunity. Applied and Environmental Microbiology, 85(11), 1–18. https://doi.org/10.1128/AEM.00371-19

Sawant, A. A., Casavant, N. C., Call, D. R., & Besser, T. E. (2011). Proximity-dependent inhibition in Escherichia coli isolates from cattle. Applied and Environmental Microbiology, 77(7), 2345–2351. https://doi.org/10.1128/AEM.03150-09

Zhao, Z., Orfe, L. H., Liu, J., Lu, S. Y., Besser, T. E., & Call, D. R. (2017). Microcin PDI regulation and proteolytic cleavage are unique among known microcins. Scientific Reports, 7(February), 1–14. https://doi.org/10.1038/srep42529

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 1498
  Illegal NheI site found at 1521
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]