Composite

Part:BBa_K3409012

Designed by: Albane Mabro   Group: iGEM20_Ionis_Paris   (2020-10-17)
Revision as of 07:11, 19 October 2020 by Albane Mabro (Talk | contribs)


Microcin PDI cluster gene for McpB

Contains the elements necessary to express the fourth gene from the Microcin PDI gene cluster (mcpD: a homolog of hlyD, coding for part of the secretion system). The sequence is organized as follows: an RBS (BBa_J1109), the CDS for McpB (BBa_K3409007). For appropriate secretion, it must be downstream of BBa_K3409012, which contains the CDS for McpD as well as a promoter (BBa_J23118) needed for transcription of McpB.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None