Device
Part:BBa_K3629017:Design
Designed by: Sravya Kakumanu Group: iGEM20_Calgary (2020-10-18)
T. reesei EGII expression construct with gibson homology sequences and FLAG tag
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2835
Illegal EcoRI site found at 2698
Illegal SpeI site found at 749 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal EcoRI site found at 2698
Illegal NheI site found at 68
Illegal NheI site found at 810
Illegal SpeI site found at 749
Illegal SpeI site found at 2836
Illegal PstI site found at 2850
Illegal NotI site found at 7
Illegal NotI site found at 2843 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal EcoRI site found at 2698
Illegal BamHI site found at 2786
Illegal XhoI site found at 125 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2836
Illegal EcoRI site found at 2698
Illegal SpeI site found at 749 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal EcoRI site found at 2698
Illegal XbaI site found at 16
Illegal SpeI site found at 749
Illegal SpeI site found at 2836
Illegal PstI site found at 2850
Illegal NgoMIV site found at 1496
Illegal NgoMIV site found at 2030
Illegal NgoMIV site found at 2348 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
x
Source
The coding sequence of the EGII is from Trichoderma reesei, however it has been codon-optimized for optimal activity in Yarrowia lipolytica. The promoter and terminator sequences are from wild-type Y. lipolytica.