Device

Part:BBa_K3629015:Design

Designed by: Sravya Kakumanu   Group: iGEM20_Calgary   (2020-10-17)
Revision as of 02:55, 19 October 2020 by Sravyakakumanu (Talk | contribs) (→‎Design Notes)


Nourseothricin resistance expression construct with gibson homology sequences


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1590
    Illegal EcoRI site found at 1449
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1449
    Illegal NheI site found at 1584
    Illegal SpeI site found at 1591
    Illegal PstI site found at 1605
    Illegal NotI site found at 7
    Illegal NotI site found at 1598
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1449
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1591
    Illegal EcoRI site found at 1449
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 1449
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1591
    Illegal PstI site found at 1605
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is an EcoRI site within coding sequence and XRP2 terminator making this part RFC10 incompatible. However we added the BioBrick prefix and suffix so that the other enzymes (NotI, XbaI, SpeI, and PstI) can be used to clone this part into an iGEM plasmid or another plasmid. This part can also be cloned through RFC1000 assembly.

Source

The coding sequence (BBa_K3629011) for the Nourseothricin resistance gene comes from Streptomyces noursei

References