Regulatory

Part:BBa_K3629002:Design

Designed by: Sravya Kakumanu   Group: iGEM20_Calgary   (2020-10-11)
Revision as of 02:59, 19 October 2020 by Sravyakakumanu (Talk | contribs) (→‎Design Notes)


Yarrowia lipolytica EXP Promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1025
    Illegal SpeI site found at 602
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 663
    Illegal SpeI site found at 602
    Illegal SpeI site found at 1026
    Illegal PstI site found at 1040
    Illegal NotI site found at 7
    Illegal NotI site found at 1033
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1026
    Illegal SpeI site found at 602
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 602
    Illegal SpeI site found at 1026
    Illegal PstI site found at 1040
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is a SpeI site within this promoter sequence, making it RFC10 incompatible. However, we added the BioBrick prefix and suffix so that the other enzymes (NotI, XbaI, SpeI, and PstI) can be used to clone this part into an iGEM plasmid or another plasmid. This part can also be cloned through RFC1000 assembly.

Source

From genomic sequence of wild-type Y. lipolytica strain CLIB122.

References