Terminator

Part:BBa_K3629004

Designed by: Sravya Kakumanu   Group: iGEM20_Calgary   (2020-10-11)
Revision as of 02:49, 19 October 2020 by Sravyakakumanu (Talk | contribs) (→‎Sequence and Features)


Yarrowia lipolytica XPR2 terminator

Terminator sequence from the Yarrowia lipolytica alkaline extracellular protease XRP2 gene.


Usage and Biology

Design

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 271
    Illegal EcoRI site found at 265
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 265
    Illegal SpeI site found at 272
    Illegal PstI site found at 286
    Illegal NotI site found at 7
    Illegal NotI site found at 279
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 265
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 272
    Illegal EcoRI site found at 265
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 265
    Illegal XbaI site found at 16
    Illegal SpeI site found at 272
    Illegal PstI site found at 286
  • 1000
    COMPATIBLE WITH RFC[1000]

This part contains an EcoRI restriction site making it RFC10 incompatible, however the other enzymes (NotI, XbaI, SpeI, and PstI) are not present in the sequence.

References

[edit]
Categories
Parameters
None