Device

Part:BBa_K3629014:Design

Designed by: Sravya Kakumanu   Group: iGEM20_Calgary   (2020-10-18)
Revision as of 07:15, 18 October 2020 by Sravyakakumanu (Talk | contribs) (→‎Source)


N. crassa CBHI expression construct with gibson homology sequences and Myc tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2751
    Illegal EcoRI site found at 1054
    Illegal EcoRI site found at 2570
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1054
    Illegal EcoRI site found at 2570
    Illegal NheI site found at 75
    Illegal SpeI site found at 2752
    Illegal PstI site found at 2766
    Illegal NotI site found at 7
    Illegal NotI site found at 2759
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1054
    Illegal EcoRI site found at 2570
    Illegal BglII site found at 1343
    Illegal BglII site found at 1703
    Illegal BamHI site found at 2700
    Illegal XhoI site found at 2745
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2752
    Illegal EcoRI site found at 1054
    Illegal EcoRI site found at 2570
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 1054
    Illegal EcoRI site found at 2570
    Illegal XbaI site found at 16
    Illegal SpeI site found at 2752
    Illegal PstI site found at 2766
    Illegal AgeI site found at 2211
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

x


Source

The coding sequence of the CBHI is from Neurospora crassa, however it has been codon-optimized for optimal activity in Yarrowia lipolytica. The promoter and terminator sequences are from wild-type Y. lipolytica.

References