Generator

Part:BBa_K150004

Designed by: Christian Moritz, Maximilian Hoerner, Anna Stoeckl, Yin Cai, Stephen Kraemer   Group: iGEM08_Heidelberg   (2008-10-21)
Revision as of 16:30, 28 October 2008 by Christian (Talk | contribs)

lambda cI generator

Lambda cI generator consisting of a strong constitutive promotor (BBa_J23101), a ribosome binding site (BBa_B0032), a lambda cI coding sequence (BBa_K150001) and a double terminator (BBa_B0015). The lambda cI coding sequence was optimized for high expression levels in E. coli

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 569
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



bla

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Categories
Parameters
None