Protein_Domain

Part:BBa_K103014:Design

Designed by: Michael Lower   Group: iGEM08_Warsaw   (2008-10-09)
Revision as of 14:23, 28 October 2008 by Smaegol (Talk | contribs) (→‎Design Notes)

OmpA_linker_alpha_linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1042
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Preparation of BBa_K103014 is in details described [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=20_October_2008&arg1=21_October_2008&arg2=22_October_2008&name=Preparation%20of%20OmpA_linker_alpha_linker%20(BBa_K103014) here] (entries from Univeristy of Warsaw 2008 iGEM team notebook).

BBa_K103006 was digested with EcoRI and SacI and ligated into pSB2K3 standard BioBrick vector (pSB2K3 with SacI site obtained by EcoRI/SacI digest of pSB2K3 carrying BBa_K103017 part)

Source

References