Coding

Part:BBa_K3394003

Designed by: Talia Shaler   Group: iGEM20_BGU-Israel   (2020-10-11)
Revision as of 08:12, 11 October 2020 by Registry (Talk | contribs)

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Glycine-serine (GS) linker

GS linker represents a glycine-serine linker, which is used for the separation of two domains in such way that they are physically connected but the original functions remain.

We decided to include GS linker in our design to keep the catalytic domain of endo5a separate from the cellulose-binding domain (CBD).

We chose this specific 10 amino-acid linker (GGGGSGGGGS) because it was used in a previous study to fuse endo5a to another domain.

NCBI Genbank number for the GS linker sequence linked to the cellulase endo5a is HQ657205.1.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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