Protein_Domain

Part:BBa_K3373006:Design

Designed by: Antonin Chevenier   Group: iGEM20_Sorbonne_U_Paris   (2020-10-17)
Revision as of 15:58, 17 October 2020 by Registry (Talk | contribs)

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GSAT-linker codon optimized for C. reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 21
    Illegal NgoMIV site found at 81
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The peptide linker is standardized in the Phytobrick standard for the C. reinhardtii MoClo Kit at the B4 (C-terminus coding region) position by adding BbsI sites and the corresponding fusion sites on both ends.


Source

Freiburg Bioware 2010 iGEM team (BBa_K404301)

References