Coding

Part:BBa_K3398003:Design

Designed by: MingXiao Wei   Group: iGEM20_ZJU-China   (2020-10-17)
Revision as of 06:51, 17 October 2020 by Lanyouyoudelv (Talk | contribs) (→‎Source)


mamC_ZZ fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 36


Design Notes

The main consideration is about how to obtain the functionally correct proteins from E.coli BL21,so we employed codon optimization to the sequence of the fusion protein. Another issue to consider is the linkage sequence between mamC and ZZ, which is refer to a recent research working on constructing a similar product as ours.


Source

The sequence of mamC comes from the genome sequence of Magnetospirillum gryphiswaldense MSR-1 and ZZ is the immunoglobulin-binding domain of staphylococcal protein A from the cell wall of Staphylococcus aureus.Both sequence were codon optimized to maximinze the expression in E.coli.For mamC (GeneBank AVM72836.1),you can visit https://www.ncbi.nlm.nih.gov/protein/AVM72836.1,and for ZZ(GeneBank:M74186.1),you can visit https://www.ncbi.nlm.nih.gov/nuccore/M74186.1 for more detailed information.

References