RNA

Part:BBa_K115001

Designed by: O.M.J.A. Stassen   Group: iGEM08_TUDelft   (2008-07-15)
Revision as of 12:52, 28 October 2008 by Bavandenberg (Talk | contribs)

RNA thermometer (ROSE)

An [http://2008.igem.org/Team:TUDelft/Temperature_analysis#RNA_thermometer RNA thermometer] that can be used for temperature sensitive post-transcriptional regulation which is based on a RNA thermometer from Bradyrhizobium japonicum that initiates translation at 42°C.

The image shows the secondary structure of the part after ligation to a protein coding part, as predicted by [http://rna.tbi.univie.ac.at/cgi-bin/RNAfold.cgi RNAfold]. Notice that the 3' end, including the scar and the start codon, does not belong to the part. The blue region is the Shine Dalgarno sequence which is the ribosome binding site. The nucleotides with a blue circle are the nucleotides that had to be changed in order to get the desired secondary structure after introduction of the scar.

BBa K115001 ss.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None