Reporter

Part:BBa_K3522004:Design

Designed by: Kong Yangyang   Group: iGEM20_NOFLS_YZ   (2020-10-16)
Revision as of 15:42, 16 October 2020 by Kimmy2020 (Talk | contribs) (→‎Source)


SV40 pro-F luc- SV40 polyA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1705
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to meet the typeIIS standard, we made a synonymous mutation of Glu271Glu to remove SapI restriction site.


Source

The coding sequence of firefly luciferase comes from the genomic sequence of photinus pyralis.

References