Other
TIIS ter
Part:BBa_K3425020
Designed by: Núria Garriga Alonso, Björn Ancker Persson, Tereza Hubáčková, Dorottya Marko, Hui Yu Group: iGEM20_UofUppsala (2020-10-03)
iGEM Type IIS standard terminator template
This part is the constitutive promoter BBa_J23105 adopted to the iGEM Type IIS standard (RFC1000). The BBa_J23105 sequence is flanked by the Type IIS prefix and suffix which allows cloning into the Level 0 backbone (pSB1C00). BBa_J23105 is part of the Anderson promoter family and its relative strength is 0.24. BBa_K3425017 can be ordered as two overlapping primers, see iGEM Type IIS guide.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 32
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 5
Illegal SapI.rc site found at 39
[edit]
Categories
Parameters
//collections/typeiis/uppsala
//collections/typeiis/uppsala/templates
//collections/typeiis/uppsala/templates
None |