Coding
Part:BBa_K3409006:Design
Designed by: Albane Mabro Group: iGEM20_Ionis_Paris (2020-10-16)
mcpD
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Did not contain any illegal restriction sites, no codon optimization was necessary.
Source
Entire gene cluster found on GenBank (KJ563250.1): https://www.ncbi.nlm.nih.gov/nuccore/700369833. Specific sequence for mcpD found on Genbank (AIU94003.1): https://www.ncbi.nlm.nih.gov/protein/700369840. Initially identified in cattle E. coli Strain SSuT-25 plasmid in 2009.