Composite

Part:BBa_K3610044:Design

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-10-10)
Revision as of 20:55, 15 October 2020 by Jtrott (Talk | contribs) (→‎Design Notes)


EFR ectodomain / SmallBit NanoLuc for C. reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 93
    Illegal NheI site found at 235
    Illegal NheI site found at 1165
    Illegal NheI site found at 2083
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 148
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1751
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization was done with SnapGene

Source

BBa_K2136017, BBa_K3610012, BBa_K3610008, BBa_M10019, BBa_K416001, BBa_K3610017

References