Composite

Part:BBa_K3610041

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-10-10)
Revision as of 19:00, 10 October 2020 by Registry (Talk | contribs)

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EFR ectodomain / mCherry N-terminal for C. reinhardtii

This part includes the ectodomain of the plant pattern recognition receptor EFR fused to the N-terminal domain of the split-mCherry protein. The sequence for the receptor and the mCherry protein have been codon optimized for expression in C. reinhardtii. To ensure localization at the membrane, this part further contains the sequence for the signal peptide SP7 from C. reinhardtii and the self-cleaving protein from the foot and mouth virus.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 93
    Illegal NheI site found at 235
    Illegal NheI site found at 1165
    Illegal NheI site found at 2083
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 148
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1751
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None