Protein_Domain

Part:BBa_K103013

Designed by: Michael Lower   Group: iGEM08_Warsaw   (2008-10-09)
Revision as of 00:42, 28 October 2008 by Smaegol (Talk | contribs)

linker fused to omega fragment of TEM beta-lactamase



Usage and Biology

  • beta fragment of the split enzyme TEM beta-lactamase
  • beta-lactamase activity can be recovered when halves are brought in close proximity by e.g. fusion to interacting domains [http://www.ncbi.nlm.nih.gov/pubmed/17005637?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum]
  • works with second half of beta lactamase (part BBa_K103009)
  • contains linker module that allows proper binding of fusion partners
  • This part uses our NdeI/SacI/BamHI fusion cloning substandard
 Linkermoega.jpg

This protein structure was predicted using threading and protein fold prediction and may differ from actual one.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 190


[edit]
Categories
//cds/enzyme
//function/reporter
Parameters
protein