Regulatory
Lac operat

Part:BBa_K079017

Designed by: Francesca Ceroni   Group: iGEM08_Bologna   (2008-10-24)
Revision as of 12:12, 28 October 2008 by Mavi.amaduzzi (Talk | contribs)

Lac symmetric - operator library member

This parts have been extracted from the Lac Operator Library.

Regulatory Elements position in natural Lac Operon

K079018: Lac O1 is the strongest wild type operator sequence in Lac Operon. In the natural system is located right after the -10 consensus sequence.

K079019: LacO2 is the intermediate affinity operator site, located 400 bp downstream of O1. In natural Lac Operon is responsible for Dna Looping, enanching repression and cooperativity of Lac system.

K079017: SymL or Oid is a perfectly symmetric Lac operator synthetically designed to maximize operator-repressor interaction. Its binding is about 10-fold tighter than the O1 natural sequence





Single Lac Operators Repression Strength defined as Unepressed versus Repressed LacZ Activity ratio

This subset of operator sequences has been chosen since it allows to cover a wide affinity and repression range














Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None