Part:BBa_K3610034
BAK1 ectodomain / mCherry N-terminal
This part contains the sequence for the ectodomain of the plant surface receptor BAK1 fused to the N-terminal part of a split-mCherry protein. Additionally, instead of the signal peptide native to the plant receptor, there is the secretion signal of the alpha factor from yeast at the N-terminal domain of the receptor, replacing the original signal sequence.
Biology and Usage
BAK1
The BRI1-associated receptor kinase (BAK1) is a leucin-rich repeat receptor kinase (LRR-RK) which interacts with multiple other LRR-RKs with different functions in hormone signalling and defense response. BAK1 localizes at the plasma membrane and the endosome. The BAK1 protein forms a structure with an extracellular domain with leucin-rich repeats, a single pass transmembrane domain and an intracellular domain with a kinase function.
Among others, BAK1 interacts with the LRR-RKs EF-Tu receptor (EFR), Flagellin sensing 2 (FLS2) and cold-shock protein receptor (CORE), all of which are pathogen recognition receptors (PRR) in brassicaceae plants. Upon binding of a microbe-associated molecular pattern at the LRR domain of the PRR, BAK1 forms a heterodimer with the PRR which triggers a phosphorylation cascade, leading to upregulation of defense mechanisms.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 849
Illegal PstI site found at 894 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 849
Illegal PstI site found at 894 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 849
Illegal PstI site found at 894 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 849
Illegal PstI site found at 894 - 1000COMPATIBLE WITH RFC[1000]
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