Part:BBa_K3610032

BAK1 ectodomain / YFP

This part contains the ectodomain of the plant cell surface receptor from A. thaliana fused to a yellow fluorescent protein. This part lacks the natural N-terminal signal sequence but instead uses the signal sequence from the alpha-Factor from yeast.

Usage and Biology

The BRI1-associated receptor kinase (BAK1) is a leucin-rich repeat receptor kinase (LRR-RK) which interacts with multiple other LRR-RKs with different functions in hormone signalling and defense response. BAK1 localizes at the plasma membrane and the endosome. The BAK1 protein forms a structure with an extracellular domain with leucin-rich repeats, a single pass transmembrane domain and an intracellular domain with a kinase function.

Among others, BAK1 interacts with the LRR-RKs EF-Tu receptor (EFR), Flagellin sensing 2 (FLS2) and cold-shock protein receptor (CORE), all of which are pathogen recognition receptors (PRR) in brassicaceae plants. Upon binding of a microbe-associated molecular pattern at the LRR domain of the PRR, BAK1 forms a heterodimer with the PRR which triggers a phosphorylation cascade, leading to upregulation of defense mechanisms.

For dimerization with a coreceptor,

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 867
  Illegal PstI site found at 912
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 867
  Illegal PstI site found at 912
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 867
  Illegal PstI site found at 912
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 867
  Illegal PstI site found at 912
  
• 1000
  COMPATIBLE WITH RFC[1000]