RBS

Part:BBa_K3425033:Design

Designed by: Tereza Hubackova   Group: iGEM20_UofUppsala   (2020-10-13)
Revision as of 10:19, 14 October 2020 by Tereza H (Talk | contribs) (→‎Design Notes)


iGEM Type IIS pSB1K03-DY


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The spacer provides aligned spacing (the distance between RBS and ATG) of 10 nucleotides when Type IIS iGEM Standard assembly is used.

Source

iGEM Registry of Standard Parts BBa_B0030

References

For aligned spacing refer:

Ma, J., Campbell, A., and Karlin, S. (2002) Correlations between Shine-Dalgarno Sequences and Gene Features Such as Predicted Expression Levels and Operon Structures. J. Bacteriol. 184, 5733–5745