Device
Part:BBa_K3629023
Designed by: Arshia Mostoufi Group: iGEM20_Calgary (2020-10-13)
2-isopropylmalate synthase (LEU4) overexpression construct with Gibson homology
LEU4 expression construct with Gibson homology, TEF promoter and double XPR2 terminator, and gPCR primer for Yarrowia lipolytica
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 339
Illegal EcoRI site found at 2875
Illegal SpeI site found at 569
Illegal PstI site found at 534
Illegal PstI site found at 986
Illegal PstI site found at 2144 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 339
Illegal EcoRI site found at 2875
Illegal NheI site found at 91
Illegal SpeI site found at 569
Illegal PstI site found at 534
Illegal PstI site found at 986
Illegal PstI site found at 2144 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 339
Illegal EcoRI site found at 2875
Illegal BglII site found at 1629
Illegal XhoI site found at 1329
Illegal XhoI site found at 1878
Illegal XhoI site found at 2136 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 339
Illegal EcoRI site found at 2875
Illegal SpeI site found at 569
Illegal PstI site found at 534
Illegal PstI site found at 986
Illegal PstI site found at 2144 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 339
Illegal EcoRI site found at 2875
Illegal SpeI site found at 569
Illegal PstI site found at 534
Illegal PstI site found at 986
Illegal PstI site found at 2144 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |