Part:BBa_K3425033

iGEM Type IIS pSB1K03-DY

This part (BBa_K3425025) is a strong RBS (BBa_B0030) modified for Type IIS iGEM Standard assembly (RFC 1000) by adding five nucleotides (TAGTA) to the end of the sequence.

Five to six nucleotides is the optimal distance between the RBS and the ATG start codon for E. coli (see https://parts.igem.org/Ribosome_Binding_Sites/Design for more general information). Whereas Biobrick assembly generates six-nucleotide scar, Type IIS iGEM Standard assembly generates only one-nucleotide scar between RBS and ATG. To keep the distance optimal, five nucleotides were added to the BBa_B0030 for its use in Type IIS assembly.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]