Plasmid
pUPD2

Part:BBa_K3505007:Design

Designed by: Venetios Michelioudakis   Group: iGEM20_Thessaly   (2020-10-13)
Revision as of 11:10, 13 October 2020 by Registry (Talk | contribs)

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pUPD2 for Golden Braid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 260
    Illegal EcoRI site found at 2674
    Illegal XbaI site found at 233
    Illegal SpeI site found at 627
    Illegal PstI site found at 221
    Illegal PstI site found at 641
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 260
    Illegal EcoRI site found at 2674
    Illegal SpeI site found at 627
    Illegal PstI site found at 221
    Illegal PstI site found at 641
    Illegal NotI site found at 634
    Illegal NotI site found at 2680
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 260
    Illegal EcoRI site found at 2674
    Illegal BamHI site found at 239
    Illegal XhoI site found at 1658
    Illegal XhoI site found at 2550
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 627
    Illegal EcoRI site found at 260
    Illegal EcoRI site found at 2674
    Illegal XbaI site found at 233
    Illegal PstI site found at 221
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 260
    Illegal EcoRI site found at 2674
    Illegal XbaI site found at 233
    Illegal SpeI site found at 627
    Illegal PstI site found at 221
    Illegal PstI site found at 641
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 14
    Illegal BsaI.rc site found at 611


Design Notes

gb venetiou


Source

pUPD2 is derived from iGEM-borne pSB1C3 [1]

References