DNA

Part:BBa_K3570009

Designed by: Anton Mykhailiuk   Group: iGEM20_Toulouse_INSA-UPS   (2020-10-01)
Revision as of 19:05, 12 October 2020 by Antonmykhailiuk (Talk | contribs)


HO upstream homologous sequence

Usage

HO upstream homologous arm used together with HO downstream (BBa_K3570010). When HO upstream put to 5' of the biobrick together with HO downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do homologous recombination within the Homothallic switching endonuclease (HO) gene.

Experiments

Source

  • [2]S. cerevisiae genome, chromosome IV, HO gene. GenBank: CP046084.1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None