Coding
Part:BBa_K3629005:Design
Designed by: Sravya Kakumanu Group: iGEM20_Calgary (2020-10-11)
Revision as of 05:09, 12 October 2020 by Sravyakakumanu (Talk | contribs)
Modified Penicillium funiculosum CBHI with 6X His tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 564
Illegal BamHI site found at 1048 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 46
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Optimized for expression and function in Yarrowia lipolytica.
Source
Modified genomic sequence of cellobiohydrolase I from Penicillium funiculosum.