Protein_Domain
Part:BBa_K3629003
Designed by: Sravya Kakumanu Group: iGEM20_Calgary (2020-10-10)
Revision as of 23:14, 11 October 2020 by Sravyakakumanu (Talk | contribs)
Yarrowia lipolytica EXP Promoter
The part contains wild-type TEF1 promoter from Yarrowia lipolytica and the first 42nts (14 codons) of the Lip2 signal peptide from Y. lipolytica (BBa_K1592000).
Upon digestion with BbsI and this part can be assembled into a destination vector (containing BBa_K3629012) with a coding sequence that contains the last 57nts (19 codons) of the Lip2 signal peptide at the 5' end by using Gibson Assembly. In our part collection, this part can be assembled with ________ into the BBa_K3629012 destination vector.
Usage and Biology
iGEM Calgary successfully created seven inducible genetic circuits for high-level production of 6GIX using various parts from our collection.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1025
Illegal SpeI site found at 602 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal NheI site found at 663
Illegal SpeI site found at 602
Illegal SpeI site found at 1026
Illegal PstI site found at 1040
Illegal NotI site found at 7
Illegal NotI site found at 1033 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1026
Illegal SpeI site found at 602 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 602
Illegal SpeI site found at 1026
Illegal PstI site found at 1040 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |