Coding
NSP2

Part:BBa_K3504001

Designed by: Ahmed Wael   Group: iGEM20_AFCM-Egypt   (2020-09-19)
Revision as of 18:23, 9 October 2020 by Ahmed Wael (Talk | contribs) (→‎Improvements)


nSp2 Protease-Eastern equine encephalitis virus

Part Description

Nsp2 is one of four non structural proteins that together forms the main complex responsible for the synthesis positive-sense viral RNAs, results in the synthesis of both the genomic and subgenomic RNAs, of which the subgenomic RNA is produced in excess of the viral genome. Which allows the virus to self-replicate into millions of copies of the virus.

Usage

Characterization

Improvements

Using information in literature we were able to increase the replicon cloning and functional ability by adding Q739L Mutation to NSP2

Figure 1. Q739L Mutation in Nsp2.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 487
    Illegal SpeI site found at 435
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 487
    Illegal SpeI site found at 435
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 487
    Illegal BglII site found at 609
    Illegal BamHI site found at 471
    Illegal BamHI site found at 834
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 487
    Illegal SpeI site found at 435
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 487
    Illegal SpeI site found at 435
    Illegal NgoMIV site found at 1276
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 739
    Illegal BsaI.rc site found at 1386


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Categories
Parameters
None