RNA

Part:BBa_K3380101:Design

Designed by: Ying Yang, Alexandru Popov   Group: iGEM20_Edinburgh   (2020-10-07)
Revision as of 08:54, 10 October 2020 by Alexandru popov (Talk | contribs)


F30 Upstream RNA aptamer scaffold


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We ordered the synthesized sequence. Originally ordered sequence: 5’ TCCCttgccatgtgtatgtggg 3’ with TCCC being a linker, to facilitate further manipulations (swapping of the part or attaching to different promoters).


Source

Reengineered from a natural three-way junction structure.


References

Filonov, G.S., Kam, C.W., Song, W. and Jaffrey, S.R., 2015. In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies. Chemistry & biology, 22(5), pp.649-660.