Coding
NSP3

Part:BBa_K3504002

Designed by: Ahmed Wael   Group: iGEM20_AFCM-Egypt   (2020-09-19)
Revision as of 11:21, 19 September 2020 by Ahmed Wael (Talk | contribs)


nSP3-Eastern equine encephalitis virus

Nsp3 is one of four non structural proteins that together forms the main complex responsible for the synthesis positive-sense viral RNAs, results in the synthesis of both the genomic and subgenomic RNAs, of which the subgenomic RNA is produced in excess of the viral genome. Which allows the virus to self-replicate into millions of copies of the virus.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 426
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1297
    Illegal PstI site found at 426
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1461
    Illegal XhoI site found at 1522
    Illegal XhoI site found at 1563
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 426
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 426
    Illegal AgeI site found at 1002
    Illegal AgeI site found at 1371
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1560
    Illegal BsaI site found at 1578
    Illegal BsaI.rc site found at 1087


[edit]
Categories
Parameters
None