Coding

Part:BBa_K3505003:Design

Designed by: Asteria Tsapadikou , Magdalini Koroxenidou, Foteini Papadaki   Group: iGEM20_Thessaly   (2020-10-09)
Revision as of 13:34, 9 October 2020 by Registry (Talk | contribs)

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LacI-Lac repressor GB compatible with B3-B5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 and has overhangs compatible for Golden Braid cloning.


Source

E.Coli , sequence found through Addgene https://www.addgene.org/82021/

References